Abstract                 Volume:3  Issue-6  Year-2016          Original Research Articles

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In Vitro Cytotoxicity and Antibacterial Evaluation of Aqueous, Methanolic and Ethanolic Extracts of Anastatica hierochuntica against Pathogenic Bacteria
Sanad M. Al Sobeai*
Sajir College of Arts and Science, Shaqra University, Shaqra, Kingdom of Saudi Arabia                                            
*Corresponding author

The cytotoxic effect of Anastatica hierochuntica was evaluated in vitro using Vero cell lines, characterized by identification of cellular alterations microscopically and cellular viability colorimetrically. The mean CC50 value of methanol, ethanol, and aqueous extract were lower than 1000 µg/ml. The antibacterial activity of ethanol, methanol and aqueous extracts of A. hierochuntica was evaluated using agar well diffusion method against 10 bacterial pathogens. All the extracts showed substantial antibacterial activity against the selected pathogens. The methanolic extract showed highest inhibition zone followed by the aqueous and ethanolic extract. Among the bacterial pathogens, L. monocytogenes was more resistant towards and ethanol extract. Although, other tested bacteria were susceptible towards aqueous ethanol and methanol extract. However, methanol extract was effective against the tested pathogens, when compared with the other extracts. The minimum inhibitory concentrations (MIC) ranged from 15-50 mg/ml to inhibit the growth of the tested bacteria. The results show that the plant contains bioactive components which can inhibit the bacterial pathogens which can be further purified and used against the multi-drug resistant bacteria.

Keywords: Anastatica hierochuntica,Antibacterial activity,Cytotoxicity,Plant extracts
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How to cite this article:

Al Sobeai, S. M., 2016. In vitro cytotoxicity and antibacterial evaluation of aqueous, methanolic and ethanolic extracts of Anastatica hierochuntica against pathogenic bacteria.Int.J.Curr.Res.Biosci.Plantbiol. 3(6): 14-22. doi: http://dx.doi.org/10.20546/ijcrbp.2016.306.002