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Abstract                 Volume:12  Issue-7  Year-2025          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
Issues : 12 per year
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Provenance, Storage Condition and Diverse Pre-Treatment Effects on Seed Germination Parameters in Afzelia africana Sm. ex Pers. (Caesalpiniaceae) – A Threatened Tree Species Collected from the Lama Forest in the Southern of Benin Republic
Ahissou Séraphin Zanklan*
Département de Biologie Végétale, Faculté des Sciences et Techniques, Université d´Abomey-Calavi, 01 BP 526, Cotonou, Bénin.
*Corresponding author
Abstract:

Assessing and monitoring forest species are essential for implementing effective conservation strategies that support biodiversity. Since Beninese Afzelia populations are threatened by regression, we analyzed as a preliminary study the germination behaviour of the species to plan subsequent programs for preservation and rehabilitation. In this context and to optimize Afzelia africana seed germination as well as to overcome and remove the obstacle of tegumentary inhibition, we have in the first preliminary attempt tested several chemical pretreatments (tap and distilled water, sodium hypochlorite 5% solution, potassium nitrate solutions KNO3 2 and 5 gL-1) for different seed ages and storage temperatures in three provenances. Thus, we could determine the optimal germination conditions that can be used afterward for evaluating the germination capacity and speed of the species. Germination capacity, speed and kinetics were analyzed, and the data was submitted to ANOVA. Kruskal-Wallis nonparametric tests were performed to identify and classify the seed ages, storage conditions, treatments and accessions. The solution of KNO3 5 gL-1 allowed the best germination capacity (75-100%) in all genotypes during the first six months-ages of the seeds. The control (tap water) permitted the lowest seed germination capacity (50-65%). The germination capacity diminished to around 50% by the twelfth-months old seeds. Genotype ‘Koto’ delivered better results than Massi NC and Tchétou. The germination speed was generally weak (0.050 J-1). The same trends for the seed germination speed as for the germination capacity were observed. Both germination capacity and speed decreased with age. Seeds stored at low temperatures (8?C) germinated better than those stored at high temperatures (37?C). Interactions: Accession × Treatment, Seed age × Treatment as well as Seed storage conditions × Treatment were very significant for all estimated parameters, indicating a relatively important contribution of the seed ages, storage temperatures, treatments and provenances to the phenotypic expression.

Keywords: Afzelia africana, Germination capacity, Germination speed, Seed age, Seed storage condition, Threatened species
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How to cite this article:

Ahissou Séraphin Zanklan. 2025. Provenance, Storage Condition and Diverse Pre-Treatment Effects on Seed Germination Parameters in Afzelia africana Sm. ex Pers. (Caesalpiniaceae) – A Threatened Tree Species Collected from the Lama Forest in the Southern of Benin RepublicInt.J.Curr.Res.Biosci.Plantbiol. 12(7): 12-34. doi: https://doi.org/10.20546/ijcrbp.2025.1207.002
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