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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
Erythrocytes, abundant in humans, are susceptible to oxidative stress, leading to membrane damage and hemolysis. This condition is prevalent in diseases like β thalasemmia, Glucose 6 dehydrogenase deficiency, and malaria. Six medicinal plants such as bark of Albizia lebbeck, endocarp of Beta vulgaris, Fruit skin of Vitis vinifera, Bark of Thespesia populnea, Fruit epicarp of Punica granatum and Leaves of Euphorbia hirta were investigated and evaluated for free radical scavenging activity and erythrocyte protective activity using their ethanolic extracts. Then, DPPH assay was carried out to all these six ethanolic extracts to study their antioxidant activity. The six ethanolic extracts were employed to prevent erythrocyte membrane oxidative damage caused by 2,2-azobis (2- amidinopropane) dihydrochloride (AAPH) in an ex vivo model. Punica granatum exhibited the highest scavenging activity (70.23±0.38%) compared to the other plants studied. Among the selected plants, Albizia lebbeck bark showed the highest levels of catalase (51.34±0.54%) and superoxide dismutase (SOD) (51.66±0.56%). The fruit epicarp of Punica granatum demonstrated the greatest inhibition of lipid peroxidation (72.66±0.82%). Additionally, it showed the highest percentage of free thiol levels (glutathione) in plasma (63.23±0.93%). All the selected plants displayed strong erythrocyte protective activity against hemolysis.
