'' '' '' ''

Abstract                 Volume:3  Issue-5  Year-2016          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorinchiefijcrbp@gmail.com

Molecular Cloning and Sequence Analysis of MADS-Box Family Gene (GbMADS6) from Ginkgo biloba L.

Yang Wan, Jia-Bao Ye, Guiyuan Wang, Weiwei Zhang*, Feng Xu and Yongling Liao

College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
*Corresponding author

Based on the results of our research group study on gingko using RNA-seq technology, a full-length cDNA sequence of MADS-box gene from Ginkgo biloba was cloned by RT-PCR, which named as GbMADS6 (GenBank accession no. KX061105). The GbMADS6 contains a 735 bp open reading frame(ORF) corresponding to a deduced protein of 245 amino acids, while the estimated isoelectric point (pI) and molecular weight of the putative protein were 5.13 and 59.67 kDa, which had a typical MADS-box domain. Homology analysis indicated that the deduced GbMADS6 protein was highly homologous to other MADS-box Proteins from different species, especially with Lolium perenne MADS4 and Sorghum bicolor MADS1 identities up to 81%. Predicted protein secondary structure showed that GbMADS6 protein contents of the structure with 51.84% of alpha helix (Hh), extended strand (Ee) of 15.10%, random coil (Cc) of 6.94% and beta turn (Tt) of 26.12%. Phylogenetic analysis showed that GbMADS6 has a closer relationship with Pinus tabuliformis MADS protein than with other MADS-box proteins. Those results suggest that GbMADS6 belongs to MADS-box super family and may be involved in regulating the development of the flower.

Keywords: Ginkgo biloba,MADS-box,Molecular cloning,Sequence analysis
Download this article as Download

How to cite this article:

Wan, Y., Ye, J.-B., Wang, G., Zhang, W., Xu, F., Liao, Y., 2016. Molecular cloning and sequence analysis of MADS-box family gene (GbMADS6) from Ginkgo biloba L.Int.J.Curr.Res.Biosci.Plantbiol. 3(5): 73-79. doi: http://dx.doi.org/10.20546/ijcrbp.2016.305.011
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.