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2Plant Biotechnology Department, Genetic Engineering Institute (GEBRI), University of Sadat City, Egypt
3Biochemistry Department, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Poland
Sufficient and promising protocol for enhancement and production of hypericin in calli cultures of Hypericum sinaicum was established. The highest value of calli cultures was produced from nodal segment and root explants respectively. MS-medium supplemented with 3 mg/l of 2,4-D gave the best results of calli production under dark condition. However, the lowest calli frequency (28 and 20%) and fresh weights (1.4 and 1.0 g/Jar) were estimated with nodal and root explants cultured on MS medium supplemented with 0.25 mg/l 2,4-D + 0.25 mg/l TDZ under light condition. Calli growth dynamics were investigated during five weeks of cultivation. Total hypericin produced by calli cultures derived from nodal segment and root explant were evaluated using HPLC.