Abstract                 Volume:7  Issue-6  Year-2020          Original Research Articles

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Biotization of Fouquieria splendens callus as protective strategy against cadmium damage
María del Rosario Espinoza-Mellado1, Marcial García-Pineda2, and Angélica Rodríguez-Dorantes3*
1Central de Microscopía, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City 11340, México
2Jardín Botánico, Facultad de Estudios Superiores, Iztacala, Universidad Autónoma de México, Los Reyes Iztacala s/n,
México City 54090, México
3Laboratorio de Fisiología Vegetal, Departamento de Botánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City 11340, México
*Corresponding author
Abstract:

Biotization’’ defined as a metabolic response of plant cells to microbial inoculants. There are some reports that this process has employed plant growth promoting bacteria to establishing an artificial symbiosis between them and tissue culture of plants. The aim of this work was analyze the protecting effect against cadmium damage by biotization of Fouquieria splendens callus with two endophyte bacterium. The effect of plant growth promoting bacteria were considered to be highly specific with respect to plant and bacterial genotypic combination; thus, Kocuria rhizophila strain Fs7 biotization does not showed a good response compared to Staphylococcus pasteuri strain Fs3, because there was an inconsistent growth of F. splendens callus. The results showed that there were also several factors influencing the growth and differentiation of callus, like the quality and especially the age of the callus employed. This study reports the positive biotization response of F. splendens callus with S. pasteuri strain Fs3 and it was able to protect callus against cadmium under in vitro conditions. This available strategy of biotization with the endophyte could be recommended as an adequate biostimulant and protective bacteria.

Keywords: Artificial symbiosis,Biotization,Cadmium,Callus culture,Endophytes
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How to cite this article:

Espinoza-Mellado, M. R., García-Pineda, M., Rodríguez-Dorantes, A., 2020. Biotization of Fouquieria splendens callus as protective strategy against cadmium damage.Int.J.Curr.Res.Biosci.Plantbiol. 7(6): 1-12. doi: https://doi.org/10.20546/ijcrbp.2020.706.001