Abstract                 Volume:7  Issue-5  Year-2020          Original Research Articles

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Molecular characterization of Pterocarpus erinaceus Poir. (Fabaceae) of Côte d'Ivoire using RAPD markers
Koudougnon Alice Estère GOBA1*, Léonie Clémence KOUONON1,
Kouamé Guillaume KOFFI1, Adjoumani KOFFI2, Bi Irié Ghislain TRE1
and Raoul Sylvère SIE1
1Université Nangui Abrogoua, Pôle de recherche Production Végétale, 02 BP 801 Abidjan 02, Côte d’Ivoire
2Ecole Normale Supérieure d’Abidjan, 08 BP 10 Abidjan 08, Côte d’Ivoire
*Corresponding author
Abstract:

Over-exploitation of Pterocarpus erinaceus, a woody species of savannah, endemic to West Africa, negatively impacts the sites of protected areas in Côte d'Ivoire. This study aims to characterize the genetic diversity of six sites using six RAPD markers in order to contribute to the implementation of optimal management and conservation plans for this species. Genomic DNA was extracted from fragments of leaflets from 144 trees following the CTAB protocol. After DNA amplification, the number of alleles ranged from 1.33 to 8.17 and the effective number of alleles ranged from 1.09 to 3.94 per site. Mean Shannon's diversity index ranged from 0.13 to 1.55 and the mean genetic diversity was estimated to 0.35. Moyenne Marahoué and Yalo were the most diversified sites with 0.64 while the lowest diversity index was recorded in Kouassi-Ndawa. In terms of genetic distance, the closest sites were Kouassi-Ndawa and Ouarigué while Moyenne Marahoué is the furthest and equidistant from Kouassi-Ndawa and Yalo. Three genetic groups were revealed. Group I is present in all the sites while group II is present in Moyenne Marahoué and group III, mainly in Yalo. For sustainable management of this species, the protection status of forests reserve Moyenne Marahoué and Yalo should be strengthened for in-situ conservation of P. erinaceus.

Keywords: Conservation,Endemic species,Genetic group,Pterocarpus erinaceus,RAPD
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How to cite this article:

Goba, K. A. E., Kouonon, L. C., Koffi, K. G., Koffi, A., Tre, B. I. G., Sie, R. S., 2020. Molecular characterization of Pterocarpus erinaceus POIR. (Fabaceae) of Côte d'Ivoire using RAPD markers.Int.J.Curr.Res.Biosci.Plantbiol. 7(5): 13-23. doi: https://doi.org/10.20546/ijcrbp.2020.705.003