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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
2Ecole Normale Supérieure d’Abidjan, 08 BP 10 Abidjan 08, Côte d’Ivoire
Over-exploitation of Pterocarpus erinaceus, a woody species of savannah, endemic to West Africa, negatively impacts the sites of protected areas in Côte d'Ivoire. This study aims to characterize the genetic diversity of six sites using six RAPD markers in order to contribute to the implementation of optimal management and conservation plans for this species. Genomic DNA was extracted from fragments of leaflets from 144 trees following the CTAB protocol. After DNA amplification, the number of alleles ranged from 1.33 to 8.17 and the effective number of alleles ranged from 1.09 to 3.94 per site. Mean Shannon's diversity index ranged from 0.13 to 1.55 and the mean genetic diversity was estimated to 0.35. Moyenne Marahoué and Yalo were the most diversified sites with 0.64 while the lowest diversity index was recorded in Kouassi-Ndawa. In terms of genetic distance, the closest sites were Kouassi-Ndawa and Ouarigué while Moyenne Marahoué is the furthest and equidistant from Kouassi-Ndawa and Yalo. Three genetic groups were revealed. Group I is present in all the sites while group II is present in Moyenne Marahoué and group III, mainly in Yalo. For sustainable management of this species, the protection status of forests reserve Moyenne Marahoué and Yalo should be strengthened for in-situ conservation of P. erinaceus.
