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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
In studies on the production of a continuously transplanted cell culture, a substrate producer of lumpy skin disease virus, primary cultures of cells from the tissues of the kidneys and testicles of lambs and goats were used. To adapt to the continuous growth in vitro, cell cultures were subjected to repeated passages by reseeding and culturing at a temperature of 37°C. The results of passivation and assessment of cell populations during cultivation showed that testicular cells have fibroblast-like morphology, a relatively large adhesive and proliferative activity. After 14 and 16 passages in the testicular cells of lambs and goats, proliferative activity decreased markedly. After further forced passages and cloning, two clonal populations of lambs testicle cells were obtained, which were distinguished by increased proliferative activity. Further passivation and adaptation to in vitro propagation can provide the desired transplanted cell line.
