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Abstract                 Volume:6  Issue-12  Year-2019          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorinchiefijcrbp@gmail.com

Isolation and enumeration of fungi and determination of contributing factors to fungal spoilage in maize (Zea mays L.) originated from east and west Shewa Zones of Oromia, Ethiopia
Temesgen A. Gelaw1 and Teshome G.  Biru2*
1Department of Biotechnology, College of Natural and Computational Science, Debre Birhan University,P.O. Box 445, Debre Birhan, Ethiopia
2Department of Applied Biology, College of Applied Natural Science, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia
*Corresponding author
Abstract:

Fungal spoilage in maize resulted in grain deterioration and mycotoxin contamination. In Ethiopia, maize production is prone to losses due to mold contamination. The purpose of this study was to isolate fungi and determine the contributing factors to fungal spoilage in Maize. 72 maize samples were collected based on incremental sampling method. Fungal isolation was done on Potato Dextrose Agar amended with 0.01% chloramphenicol. Fungal colonies were purified by sub-cultured on PDA. Morphological and microscopic characterization of the isolates was done based on fungal identification manual. From 1080 maize grains, 613 fungal isolates were recorded with 54.1% mean value where the majorities were belongs to the genus Fusarium. Grains collected from warehouse were more infected (58.88%) followed by store, open market and household. Overall, the prevalence of fungal infection in the study sites was high. Awareness creation, cumulative integrated management, monitoring, and precautionary measures are recommended.

Keywords:

Food contamination,Fungal spoilage,Maize,Mycotoxin

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How to cite this article:

Temesgen, A. G., Teshome, G. B., 2019. Isolation and enumeration of fungi and determination of contributing factors to fungal spoilage in maize (Zea mays L.) originated from east and west Shewa Zones of Oromia, Ethiopia.Int.J.Curr.Res.Biosci.Plantbiol. 6(12): 8-19. doi: https://doi.org/10.20546/ijcrbp.2019.612.002
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.