Abstract                 Volume:6  Issue-8  Year-2019          Original Research Articles

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Screening of PHB biopolymer producing Bacillus cereus from municipal sludge waste
P. Murali, K. Mamatha and N. Mathiyazhagan*
PG and Research Centre in Biotechnology, MGR College, Hosur- 635 130, Krishnagiri, Tamil Nadu, India
*Corresponding author
Abstract:

Biopolymers are the rarely produced bio products which are used as an alternative for common plastic and some microbes can even digest them. In this study we have confirmed that Bacillus cereus can produce such bio polymers. B. cereus, isolated from sludge soil sample was standalone which showed substantial PHB production (4.2 ±0.25 g/l) after 48 h of fermentation as examined by spectrophotometric analysis. Time-course (24-96 h) analysis for PHB production indicated that PHB was a growth-associated product and its accumulation significantly increased during the exponential phase and reached maximum after 48 h (5.6 ±0.25 g/l). The maximum PHB yield was achieved after 48-72 h of fermentation (6.5±0.18 g/l). The FT-IR analysis, illustrates bands at 3280.92 and 2924.09 cm−1 corresponding to aliphatic ester carbonyl C=O of R COA and C-H stretch, respectively. Other absorption bands at 1633.71 and 1533.09 cm−1, represent the presence of O-H bonding and C-H bond of aliphatic compounds, respectively. The band at 1633.71 cm−1 considered as diagnostic signal for PHB. The band found at 1,380.17 cm−1 is assigned to symmetric wagging of CH3 groups. The 1H-NMR spectra showed the presence of three signals, it’s the characteristic of the polymer of PHB.

Keywords: Bacillus cereus Biopolymer Fermentation PHB Phosphate
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How to cite this article:

Murali, P., Mamatha, K., Mathiyazhagan, N., 2019. Screening of PHB biopolymer producing Bacillus cereus from municipal sludge waste.Int.J.Curr.Res.Biosci.Plantbiol. 6(8): 13-20. doi: https://doi.org/10.20546/ijcrbp.2019.608.003