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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
2Institute of Integrated & Honors Studies (IIHS), Kurukshetra University, Kurukshetra- 136 119, Haryana, India
3Principal Scientist, ABC division, ICAR-National Dairy Research Institute, Karnal, Haryana- 132 001, India
The protein tyrosine phosphorylation is considered as a hallmark event of capacitation in most of the mammalian spermatozoa. The induction of premature capacitation is one of the detrimental effects of cryopreservation. In present study, we have compared the tyrosine phosphorylation of proteins in fresh and cryopreserved buffalo spermatozoa in egg yolk tris (without additives; taken as control), soya milk tris (without additives) and soya milk tris extender with additives like Taurine and Trehalose. The tyrosine phosphoproteins were detected by immunoblotting using Clone PT-154 as primary antibody and a HRP-conjugated secondary antibody followed by chemiluminescence detection. Ten tyrosine phosphoproteins were detected in cryopreserved buffalo spermatozoa. A significant (p<0.05) decrease in tyrosine phosphorylation was observed in soya milk tris based extender. Furthermore, the supplementation of Taurine and Trehalose in soya milk extender significantly (p<0.05) reduced the level of tyrosine phosphorylation indicating the reduced cryocapacitation as compared to the egg yolk extender.
