Abstract                 Volume:6  Issue-2  Year-2019          Original Research Articles

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In vitro allelopathic activity of aqueous leaf extract of Hyptis suaveolens (L.) Poit. against growth parameters of plant seeds and in situ analysis of rhizosphere microflora
R. Pratap Chandran*, Ahlen Ig Barry2, Reshma Shaji2, J. Vineetha2, Neeraja Unnikrishnan2 and S. Aswathy2
1Department of Biotechnology and Research, K. V. M. College of Science and Technology, Kokkothamangalam P. O., Cherthala - 688583, Alappuzha District, Kerala State, India
2Department of Biotechnology, S. D.V. College of Arts and Applied Science, Sanathanapuram P.O., Kalarcode,
Alappuzha District, Kerala State, India
*Corresponding author
Abstract:

The allelopathic effect of aqueous leaf extract of Hyptis suaveolens were tested against Oryza sativa and Brassica juncea seeds and plant growth parameters such as seed germination, speed of germination, mean germination time, phytotoxicity, radicle and plumule length, seed vigour index, percentage of toxicity and relative germination ratio were studied. Different concentrations (20, 40, 60, 80 and 100 %) of H. suaveolens aqueous extracts were irrigated in test plates and the control plate was irrigated with sterile distilled water. For each treatment, three different replicates were tested with 10 seeds each. 5 ml of each of aqueous extracts were added to Petri dishes laid with filter paper every day to avoid drying out of filter paper during the course of experiment. The Petri plates were kept in a germinator (25±3˚C, 70% humidity and 12 h photoperiod) for 7 days. Germinated seeds (considered when radical emerges ≥ 1mm) were daily counted for 7 days and measurements of both radicle and plumule were noted. To evaluate the allelopathic activity against microorganism, rhizosphere soil samples were collected from four different sites from H. suaveolens and from a control non-phenolic plant, Crotalaria pallida. Soil samples were serially diluted to different concentrations and were plated on correspondingly labeled nutrient agar and potato dextrose agar plates. Analysis of rhizosphere soil of H. suaveolens and Crotalaria pallida (control plant) were also studied from four different locations and found that the bacterial and fungal count of rhizosphere soil of H. suaveolens was low when compared to C. pallida rhizosphere soil.

Keywords: Allelopathy Germination Hyptis suaveolens Inhibition Rhizosphere
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How to cite this article:

Pratap Chandran, R., Ig Barry, A., Reshma, S., Vineetha, J., Neeraja, U., Aswathy, S., 2019. In vitro allelopathic activity of aqueous leaf extract of Hyptis suaveolens (L.) Poit. against growth parameters of plant seeds and in situ analysis of rhizosphere microflora.Int.J.Curr.Res.Biosci.Plantbiol. 6(2): 61-69. doi: https://doi.org/10.20546/ijcrbp.2019.602.005