Abstract                 Volume:6  Issue-1  Year-2019          Original Research Articles

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Impacts of supplementary royal jelly on characteristics of post-thawed ram semen
Hatem ATALLA 1 *, Ahmet ESER 2, Kamber DEMİR 2, Selin YAĞCIOĞLU 2, Ramazan ARICI 2, Nur ERSOY 2, Mithat EVECEN 2, Kemal AK 2, Sema BİRLER 2 and Serhat PABUCCUOĞLU 2
1Department of Veterinary Medicine,Faculty of Agriculture and Veterinary Medicine, An Najah National University,Nablus – PALESTINE
2 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University,TR-34320 Avcılar, Istanbul – TURKEY
 
*Corresponding author
Abstract:

The motility, sperm membrane and acrosome integrity during post–thawing incubation is an indication of the usability of the semen. The maintenance of higher motility of sperm during post–thawing incubation simultaneously with the maintenance of intact cell membrane and acrosome integrity reflects a greater likelihood of their survival in the female genital tract to undergo capacitation and fertilize ova or even to be used in in vitro embryo production. The aim of this study was to elucidate the protective ability of royal jelly on motility, spermatozoa plasma membrane and integrity of acrosome during 4 hours of incubation of post-thawed ram semen in thawing media contains different concentrations of RJ (0.5, 1.0 and 2.0%). The post-thaw results revealed that 0.5% RJ supplementation to incubation media significantly improved (P< 0.05) total and progressive motility after the second hour of incubation compared to 0, 1 or 2% RJ groups; however, a non-significant difference (P> 0.05) was obtained regarding the sperm acrosome integrity across all the treatment groups. In conclusion, the structural and functional parameters of sperm during -thawing can be enhanced by supplementing the RJ in post-thawing media before used especially in IVF process and a dose of 0.5% RJ provide the best post-thaw sperm quality.

Keywords: Post-thawed Ram semen Royal Jelly
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How to cite this article:

Atalla, H., Eser, A., Demir, K., Yağcioğlu, S., Arici, R., Ersoy, N., Evecen, M., Ak, K., Birler, S., Pabuccuoğlu, S., 2019. Impacts of supplementary royal jelly on characteristics of post-thawed ram semen.Int.J.Curr.Res.Biosci.Plantbiol. 6(1): 6-11. doi: https://doi.org/10.20546/ijcrbp.2019.601.002