Abstract                 Volume:6  Issue-1  Year-2019          Original Research Articles

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Amplification of the Y1.7, Y1.8 and GAPDH genes for sex identification in human by using multiplex PCR
Maytham Abdul Kadhim Dragh1*, Talib Ahmed Jaayid2 and Zainab Sabeeh Hasan3
1 Biological Department, College of Science, University of Misan, Misan, Iraq
2 Animal Production Department, Agriculture College, University of Basrah (UoB), Basrah, Iraq
3 Animal Production Department, College of agriculture, University of Maisan, Maisan, Iraq
*Corresponding author

Pre-natal embryo sexing was done using circulatory blood of pregnant women at early stages of pregnancy, before the 4th month of pregnancy, in which 20 samples were obtained the objective of the study was to establish and evaluate multiplex PCR for detection of Y-chromosome – specific sequence of fetal DNA in maternal blood circulation during pregnancy in which identification of fetal gender become possible before the 4th month of pregnancy, this pre-test can be used to determine whether invasive prenatal diagnosis, such as amniocentesis and chorionic villi sampling should be performed on a fetus having a risk of X- linked recessive inheritance. The results of the study showed the appearance of a band with 57bp (represent the primer GAPDH) In all samples which represented the control. The second band with the 100bp (represent the primer Y1.7 and Y1.8) as well as the first band (57bp) appeared in male fetuses since it represent a gene found only on the Y chromosome. The application of a non-invasive method like multiplex PCR by using maternal blood for embryo sex identification reveals a reliable and accurate method for prenatal diagnosis.

Keywords: Amplification GAPDH Genes Multiplex PCR Prenatal diagnosis
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How to cite this article:

Abdul Kadhim Dragh, M., Ahmed Jaayid, T., Sabeeh Hasan, Z., 2019. Amplification of the Y1.7, Y1.8 and GAPDH genes for sex identification in human by using multiplex PCR.Int.J.Curr.Res.Biosci.Plantbiol. 6(1): 1-5. doi: https://doi.org/10.20546/ijcrbp.2019.601.001
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