|Online ISSN : 2349-8080
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2University College, Kurukshetra University, Kurukshetra - 136119, India
Cut flowers of Calendula officinalis L. as scapes were maintained in holding solutions of 0.1M sucrose, 20 M methyl chlorflurenol (a morphactin, MOR), 1.5 mM 8-hydroxyquinoline (8-HQ), sucrose + MOR, sucrose + 8-HQ and sucrose + MOR + 8-HQ in the laboratory under continuous light. Untreated sets were also present where scapes getting the supply of double distilled water (DDW). Combined applications of sucrose + 8-HQ and sucrose + MOR + 8-HQ were very effective in minimizing the loss of fresh weight of scapes and moisture content. These combinations and also individual sucrose treatment as well as sucrose + MOR were very useful to reduce the loss in flower diameter by maintaining hydration and turgidity. Sucrose + MOR + 8-HQ combination was also unique in exhibiting maximum rise in protein amount on fresh weight basis between 0 and 3 and 3 and 6-day and on dry weight basis between 0 and 3-day. Lesser GPOX activity in petals of flower scapes subjected to 8-HQ, MOR and sucrose treatments may suggest lesser production of H2O2. Further, used chemicals such as sucrose, MOR and 8-HQ could reduce the lipid peroxidation process in petals and the best treatment was sucrose + MOR + 8-HQ followed by sucrose + 8-HQ > sucrose + MOR > MOR> 8-HQ > sucrose > DDW.