Abstract                 Volume:5  Issue-8  Year-2018          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
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Phytoplasma Detection on Sugarcane Crops in South Sulawesi
Catur Yogo Hendro Utomo1*, Baharuddin2 and Nur Amin2
1Master Program, Department of Plant Pests and Diseases, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia
2Departement of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia
*Corresponding author
Abstract:

Sugarcane yellow leaf syndrome has been found in South Sulawesi. The aim of this study was to detect phytoplasmas associated with the symptomatic and asymptomatic sugarcane. This study included sampling both symptomatic and asymptomatic leaves, DNA extraction, detection phytoplasma using nested PCR, sequencing and confirmation using Basic Local Alighment Search Tool (BLAST) program. Detection using nested PCR shows the amplification of the 1250 bp DNA band showing the presence of phytoplasma both symptomatic and asymptomatic leaves. This means that sugarcane crop in South Sulawesi has been infected with phytoplasmas.

Keywords: Phytoplasma,Sugarcane,Yellow leaf syndrome
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How to cite this article:

Utomo, C. Y. H., Baharuddin, Nur Amin, 2018. Phytoplasma detection on sugarcane crops in South Sulawesi.Int.J.Curr.Res.Biosci.Plantbiol. 5(8): 24-28. doi: https://doi.org/10.20546/ijcrbp.2018.508.004