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In the present study the ethanol extract of Sesamum alatum Thonn. leaves were tested for antibacterial activity using disc diffusion assay followed by resazurin dye reduction test. The antibacterial activity of plant extracts was determined by resazurin dye reduction method. The dye resazurin was obtained from Sigma chemicals and prepared as 10g /l sterile water stock solution, stored in frozen at -20°C, thawed and diluted 1:10 in sterile water when required. The titreplate resazurin assay was performed in 96-well plates. Two-fold dilutions of each antibiotics and plant extracts were prepared in the test wells in complete nutrient broth, the final antibiotic concentrations being streptomycin 0.06 mg/l and tetracycline 0.12 mg/l. Twenty microlitres of each bacterial suspension was added to 180 μl of antibiotics and plant extracts containing culture medium. The minimum inhibitory concentration (MIC) of the ethanolic extracts of the leaves were rapidly assessed against five different bacterial species, viz., Micrococcus luteus (NCIM-2103), Staphylococcus epidermidis (NCIM-2493), Escherichia coli (NCIM-2065), Pseudomonas cepacia (NCIM-2106) and Bacillus megaterium (NCIM-2187). The highest antibacterial activity with least MIC values were recorded in the ethanolic leaf extracts of Sesamum alatum against the test bacteria (MIC = 62.5-250 µL). The diameter of zone of inhibition was ranged from 0.9-1.9 cm for the bacteria tested. The highest inhibition zone was observed against Escherichia coli (1.9 cm) followed by 1.6 cm for Pseudomonas cepacia and 1.4 cm for Staphylococcus epidermidis. The results indicate that the ethanolic extract of Sesamum alatum leaves has good antimicrobial activity in crude form.
Antibacterial activity,Inhibition zone,Leaf extract,Resazurin dye reduction,Sesamum alatum