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Abstract                 Volume:4  Issue-6  Year-2017          Original Research Articles

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Preliminary Phytochemical Analysis and Antibacterial Activity of Leaf Extracts of Capparis sepiaria, Euphorbia heterophylla and Tamilnadia uliginosa
B. Kalpana1 and M. Prakash2*
1Research & Development Centre, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
2Department of Microbiology, Kanchi Shri Krishna College of Arts and Science, Kilambi, Kancheepuram-631 551, Tamil Nadu, India
*Corresponding author

In the present study the ethanol and petroleum ether extracts of Capparis sepiaria, Euphorbia heterophylla and Tamilnadia uliginosa leaves were subjected to preliminary phytochemical analysis and tested for antibacterial activity using resazurin dye reduction test. The minimum inhibitory concentration (MIC) of the ethanolic and petroleum ether extracts of the leaves were rapidly assessed against five different bacterial species, viz.,  Micrococcus luteus (NCIM-2103), Staphylococcus epidermidis (NCIM-2493), Escherichia coli (NCIM-2065), Pseudomonas cepacia (NCIM-2106) and Bacillus megaterium (NCIM-2187). The highest antibacterial activity with least MIC values were recorded in the ethanolic leaf extracts of Tamilnadia uliginosa against the test bacteria (MIC = 62.5-250 µL). In petroleum ether extracts of all the plants tested, Tamilnadia uliginosa recorded enumerable activity with least MIC value ranging between 125-500 µL. The results indicate extracts of Tamilnadia uliginosa leaves has good antimicrobial activity in crude form.

Keywords: Antibacterial activity, Leaf extract, Medicinal plants,Minimum inhibitory concentration, Phytochemicals, Resazurin reduction test
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How to cite this article:

Kalpana, B., Prakash, M., 2017. Preliminary phytochemical analysis and antibacterial activity of leaf extracts of Capparis sepiaria, Euphorbia heterophylla and Tamilnadia uliginosa.Int.J.Curr.Res.Biosci.Plantbiol. 4(6): 114-120. doi: https://doi.org/10.20546/ijcrbp.2017.406.013
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