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2Department of Botany, K.T.H.M. College, Nashik, Savitribai Phule Pune University, MS, India
Dioscorea bulbifera is an important medicinal plant for novel pharmaceuticals since most of the bacterial pathogens are developing resistance against many of the currently available antimicrobial drugs. The antimicrobial activity of all the extracts of D. bulbifera against Staphylococcus aureus, Escherichia coli, Aspergillus, Pseudomons, Candida, Streptococcus pyogenes, Streptobacillus, Klebsiella pneumoniae, Bacillus megaterium, Pseudomonas fluorescens, Bacillus subtilis and Xanthomonas sp.; the agar well diffusion method (E Test) to determine MIC, MBC and Potentiation effect on Gentamicin were investigated. Using Soxhlet extraction, methanolic extracts were prepared from dried bulbs, leaves and stems of D. bulbifera which were used to evaluate their Antimicrobial Susceptibility, Synergistic effect, Phytochemical analysis, FTIR, Nutritional analysis and Antioxidant activity. Data interprets that the pattern of inhibition depends upon the plant part used, the solvent chosen, the extraction procedure, temperature, the assaying method and the organisms tested. S. aureus was found to be most suscebtible, while Xathomonas sp. was resistant to all the organic extracts. The MIC values obtained by agar well diffusion method and broth dilution method had shown little discrepancies. The combinations of extract for synergistic activity against the tested pathogens were found to be additive for bacterial pathogens and antagonistic for fungal pathogens. Combinations of stem and bulb were found to give better synergistic activity. Alkaloids, terpenoids, phenols and tannins were present in all the extracts. Stem extract was found to be better followed by bulbs and leaves. FTIR analysis showed the presence of various functional groups. Stem extract showed the maximum antioxidant activity. Nutritional analysis showed significant levels of nutrients present in bulbs. There are very scanty research papers available on the work done on leaves and stems of D. bulbifera.