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2Department of Botany, Tagore Government Arts and Science College, Puducherry- 605 008, India
Traditional medicinal herb Achyranthes aspera L. holds an important place in Siddha and Ayurvedic medicines. Plant tissue culture studies have been carried out in this plant employing various explants. However, no report is available on micropropagation through shoot tip explant. Surface sterilization using Bavistin and HgCl2, and in vitro germination procedure adapted for A. aspera provided very low level of contamination than other published reports, hitherto. Shoot tips cultured on Murashige and Skoog medium fortified with BAP at 2 mg/L produced maximum number of multiple shoots. Combinations of different concentrations of BAP with IAA did not facilitate the production of more shoots. Rooting of microshoots was successful in 2 mg/L of IBA. Vermiculite containing garden soil was suitable for hardening of somaclones.