Abstract                 Volume:4  Issue-12  Year-2017          Original Research Articles

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In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.
Muthuvel*, M. Vigneswaran and N. Jayabalan
Department of Botany, Bharathidasan University, Tiruchirappalli – 620024, Tamil Nadu, India                              
*Corresponding author
Abstract:

An efficient in vitro plant regeneration protocol was developed for Trichosanthes cucumerina L. from leaf and shoot tip explant. Explants were cultured on MS medium supplemented with 6-benzyl amino-purine (BAP) thidiazuron (TDZ) and kinetin (Kin) ranging from 0.5-3.0 mg/L alone and in combination with gibberellic acid (GA3) ranging from 0.2-1.0 mg/L and produced multiple shoots. The highest percentage of response 96.3 percentage of response in leaf and 39.33 percentage of response in shoot tip explant were noticed on 0.6 mg/L GA3 in combination with 2.5 mg/L BA. The 84.38 and 24.96  percentage of response were noticed in leaf and shoot tip explants respectively in 2.5 mg/L. The regenerated shoots were then transferred to MS medium fortified with different concentrations of indole acetic acid (IAA), 1-naphthalene acetic acid (NAA) and indole butyric acid (IBA) (0.5-3.0mg/L) alone for root induction. The maximum percentage of response 96.63 with 9.66 cm root length per shoot was obtained from 1.5 mg/L NAA. The rooted plantlets were successfully acclimatized in red soil, farmyard manure and sand mixture and subsequently established in the field with 90% survival.

Keywords: Growth hormones,Leaf explant,Multiple shoot,Shoot tip explant,Trichosanthes cucumerina
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How to cite this article:

Muthuvel, M., Vigneswaran, M., Jayabalan, N., 2017. In vitro plant regeneration through different explant of Trichosanthes cucumerina L.Int.J.Curr.Res.Biosci.Plantbiol. 4(12): 108-116. doi: https://doi.org/10.20546/ijcrbp.2017.412.009