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Endophytic fungi are a group of unexplored microorganisms with an abundant source of natural bioactive compounds of industrial and pharmaceutical importance. The current study reports a total of five different endophytic fungi viz., Phoma sp., Phomopsis sp. (brown colonies), Phomopsis sp. (white colonies), Drechslera biseptata, Nigrospora sphaerica isolates obtained from the leaf of Bauhinia sp., Delonix regia and Crotalaria sp. The highest colonization frequency was observed in Phoma sp. (75%) followed by Drechslera biseptata (61%). Extra hyphal clearing was observed for all the isolates grown on media containing glucose as the sole carbon source indicating the presence of amylase whereas Phoma sp. and Nigrospora sphaerica were able to produce laccase. Phoma sp. and Phomopsis sp. (white) were able to produce protease. The isolates also produced ammonia. Klebsiella pneumoniae and Pseudomonas aeruginosa was inhibited by Phoma sp., Phomopsis sp. (white), Phomopsis sp. (brown), Drechslera biseptata and Nigrospora sphaerica at a concentration of 5mg/ml. Proteus mirabilis was inhibited by all the isolates except Drechslera biseptata. Interspecific activities of the isolated endophytic fungi indicated Nigrospora sphaerica was the dominant organism.