Abstract                 Volume:4  Issue-10  Year-2017          Original Research Articles

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Effect of Re-irradiation on Vegetative Traits of Apple Malus domestica In Vitro
A. Abdulmajeed and Z. A. AL-Hussaini*
Department of Genetic Engineering, Agriculture, Research Directorate, Ministry of Science and Technology, P.O. Box 765, Baghdad-Iraq                                                                           
*Corresponding author

Plantlets of Apple Royal Gala were irradiated twice for Gamma rays at different doses 0, 10, 20, 25, 30, 40 Gy. Stem cuttings (with two nodes) and the leaf segments were excised, as well as the leaf segments were directly irradiate for once with the same doses. The cultures were incubated in the growth room chamber at 25°C±2 under 16 h light and 8 h dark. Data on percentage of survival %, Shoots per explant and plant height were taken. According to the percentage of survival criteria, Stem cuttings showed a high percentage (100%) for one exposing at all doses Whilst that twice exposing showed a decrease in survival reached 10 % at 30 Gy with completely failed to grow at 40 Gy. Leaf segments also showed gradually decreased in survivals at increasing doses for one and twice exposing. The irradiated leaf segments were directly exposed affected at all doses of radiation, the lowest survival was 6% at 40 Gy. The results also showed the negative effect of irradiation which was repeated twice on the number of shoots and plant height for stem cutting and leaf segments. It is clear that leaf segments which directly irradiated are affected by the reduced formation of the vegetative shoots by increasing of radiation doses.

Keywords: Gamma ray,Growth hormonresa,In vitro culture,Malus domestica,Re-irradiation
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How to cite this article:

Abdulmajeed, W. A., AL-Hussaini, Z. A., 2017. Effect of re-irradiation on vegetative traits of apple Malus domestica in vitro.Int.J.Curr.Res.Biosci.Plantbiol. 4(10): 38-43. doi: https://doi.org/10.20546/ijcrbp.2017.410.003