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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
The aim of the study is to extract lutein from microalgae (Chlorella spp. and Scenedesmus spp.) and study its anti-oxidant, anti-bacterial and anti-cancer activity. Lutein was extracted by isolating and cultivating Chlorella spp. and Scenedesmus spp. and by using Chlorella spp. powder. Chlorella spp. was cultivated in two types of media namely Chu’s 10 media and Basal Bold media (BB) media. The cultivation was carried out for a period of two months. Alkaline treatment (using KOH) was done and then followed by 3-step di-ethyl ether extraction. Lutein was extracted using three different solvents namely acetone, methanol and butanol and without any solvent but using olive oil. Test for carotenoids was done in order to confirm the presence of carotenoids. Anti- bacterial test (well agar diffusion) was done in order to find whether lutein was active against the bacterial organism (Staphylococcus spp. and E. coli.). Anti-oxidant assay {Total Anti-oxidant Capacity (TAC) and H2O2 anti-oxidant assay} was done to find the anti-oxidant activity. Anti-cancerous activity (MTT assay) was done in order to study the anti-cancer properties of lutein extracted from micro-organism. Gas Chromatography- Mass spectroscopy (GC-MS) was done to find the concentration of lutein present in the algal samples. The extraction of lutein from microalgae will provide a new source of lutein derived from micro-organisms.
