Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
2Faculty of Girl Education, Kufa University, Iraq
In order to determine the optimal radiation dose, the sensitivity of Riviera and Burren genotypes to gamma rays was tested. Cuttings exposed to gamma irradiation of 0, 5, 10, 15, 20, 25 and 30 Gy and placed in callus induction medium, which showed that 0, 5, 10 and 15 Gy were gave higher percentage reached 100% with shorter times (10-12 days) for each genotypes while 20 and 25 Gy caused reduction in callus induction and increasing the time taken for induction. Results based on callus growth criteria showed the optimal doses for mutation were 12 and 18 Gy for Burren and Riviera respectively. Irradiated and non irradiated calli were planted in medium supplemented with different salt levels (6, 8, 10, 12 dS m-1). At all salt levels, radiation decreased callus fresh weight, relative growth rate and water content and increased accumulation of K+, Na+, Cl- and proline. Ca++ and carbohydrate accumulations was not affected by radiation. In both genotypes, salinity stress decreased callus fresh weight, relative growth rate and water content and increased Na+, Cl- and proline accumulations.