Abstract                 Volume:4  Issue-7  Year-2017          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
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Aflatoxin B1 Contamination Correlation with Aspergillus flavus in Animal Feed Raw Materials Imported through the Port of Tanjung Perak Surabaya, Indonesia
Yunetta Putri Arios1*, Agustin Indrawati2 and Joko Pamungkas2
1Indonesian Agricultural Quarantine Agency, Ministry of Agriculture, Stasiun Karantina Pertanian Kelas I Merauke, Indonesia
2Department of  Animal Diseases and Veterinary Public Health, Faculty of Veterinary  Medicine, Bogor Agricultural Universty, Bogor 16680, West Java, Indonesia
*Corresponding author
Abstract:

This study aimed to measure the incidence rate of aflatoxin B1 contamination in feed raw materials (MBM and PPM) imported through Port of Tanjung Perak Surabaya, and its relation to the growth of the fungi that produce it. ELISA (Enzyme Linked Immunosorbent Assay) competitive was conducted to identify Aflatoxin B1 and followed by HPLC (High Performance Liquid Chromatography). Fungal identification was conducted as a macroscopic and microscopic observation. ELISA test showed 55 of 84 (55.95%) samples were positive for aflatoxin B1 contamination, while 3 of 7 (42.85%) samples tested by HPLC were positive. The result of fungal identification showed 1 of 12 samples positive Aspergillus flavus, and others types of fungi. AFB1 contamination in animal feed raw materials is likely to have occurred in the country of origin, whereas the growth of the fungus is due to the level of humidity and temperature during the transportation and storage process.

Keywords: Aflatoxin B1,Aspergillus flavus,Raw animal feed,Surabaya
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How to cite this article:

Arios, Y. P., Indrawati, A., Pamungkas, J., 2017. Aflatoxin B1 contamination correlation with Aspergillus flavus in animal feed raw materials imported through the Port of Tanjung Perak Surabaya, Indonesia.Int.J.Curr.Res.Biosci.Plantbiol. 4(7): 23-32. doi: https://doi.org/10.20546/ijcrbp.2017.407.003