Abstract                 Volume:4  Issue-4  Year-2017          Original Research Articles

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Assessment for the Incidence of Number of Subcultures on Genotype Stability for In Vitro Plantlets of Yam (Dioscorea spp.) Using RAPD Markers
Arnaud Agbidinoukoun1*, Antoine Abel Missihoun1, Pierre Akonde2, Hurbert Adoukonou Sagbadja1, Clément Agbangla1 and Corneille Ahanhanzo1
1Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP 526, Cotonou, Republic of Benin
2Institut National de Recherche Agronomique du Bénin (INRAB), Cotonou, Republic of Benin
*Corresponding author

The impact of the number of subcultures on the stability of yam genotype maintained in   in vitro genebank was investigated. Six accessions of yam (Ala, Bagri Kogan, Kokoro, Sossou, and Tankpanou), belonging to the complex Dioscorea cayenensis- rotundata were initiated on free-hormone MS medium and were micropropagated each four months during five subcultures. DNA was extracted both from mother plant and plantlets provided from the subcultures for each accession and was exhibited Random Amplified Polymorphic DNA (RAPD) analysis using four selected primers to detect somaclonal variation. No phenotypic variation was observed during the fifth subcultures. From the RAPD analysis of both mother plants and in vitro plantlets, no significant variation of DNA profiles was observed with the highest of the coefficient of similarity (85% to 100%) for all accessions, thus ensuring the genetic stability of the plants and regeneration of true to type plants for at least five subcultures.

Keywords: Dioscorea spp.,Genotype fidelity,In vitro genebanks,RAPD,Subcultures
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How to cite this article:

Agbidinoukoun, A., Missihoun, A. A., Akonde, P., Sagbadja, H.A., Agbangla, C., Ahanhanzo, C., 2017. Assessment for the incidence of number of subcultures on genotype stability for in vitro plantlets of yam (Dioscorea spp.) using RAPD markersInt.J.Curr.Res.Biosci.Plantbiol. 4(4): 32-38. doi: https://doi.org/10.20546/ijcrbp.2017.404.006