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Abstract                 Volume:4  Issue-4  Year-2017          Original Research Articles

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Cloning and Sequence Analysis of a Flavonol Synthase (FLS2) Gene from Ginkgo biloba
Hui Zeng, Li Yang, Hua Rong, Weiwei Zhang*, Feng Xu and Xin Zhang
College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
*Corresponding author

Flavonol Synthase, belongs to 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, was involved in flavonol biosynthesis in plants. In this study, we cloned a cDNA encoding flavonol synthase from Ginkgo biloba (designated as GbFLS2).The cDNA of GbFLS2 gene is 1251 bp and contains a 1017 bp open reading frame encoding 338 amino acids. The deduced protein of GbFLS2 displays extensive homology to FLS proteins from other plants such as Pinus radiata, Dioscorea alata, Camellia sinensis and Epimedium sagittatum. Phylogenetic analysis indicated that the GbFLS2 has a closer relationship with FLS from gymnosperm plants than from other plant species. The results suggest that GbFLS2 is a member of the 2-ODD superfamily, and it is from the same ancestor as FLS proteins of other gymnosperm plants.

Keywords: Flavonol Synthase,Gene clone,Ginkgo biloba,Sequence analysis
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How to cite this article:

Zeng, H., Yang, L., Rong, H., Zhang, W., Xu, F., Zhang, X., 2017. Cloning and sequence analysis of a Flavonol Synthase (FLS2) gene from Ginkgo biloba.Int.J.Curr.Res.Biosci.Plantbiol. 4(4): 14-19. doi: https://doi.org/10.20546/ijcrbp.2017.404.003
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