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Abstract                 Volume:4  Issue-2  Year-2017          Original Research Articles

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Sensitivity of Azotobacter chroococcum to Exogenous Nitrogen in Rice Cultivation in Terms of Nitrate Reductase Activity in Leaves and Nitrogenase Activity on the Root Surface of the Inoculated Rice Cultivar and Its Effect on Microorganism Population in Rhizosphere Soil
Mihirlal Roy1*, Sibani Saha2 and Jhuma Das3
1Tripura State Council for Science & Technology, A.R.Complex, Agartala-799 006, Tripura India
2Department of Fisheries, Government of Tripura, College Tilla, Agartala799 004, Tripura, India
3IDSP, State Health and Family Welfare Society, Agartala799 006, Tripura, India
*Corresponding author

The experiment on inoculation of Azotobacter chroococcum on cv. IR–64 of Oryza sativa L. in integration with different levels of exogenous nitrogen (urea-nitrogen) conducted on paddy soil in earthenware pots in rainy autumn season in West Tripura. The result indicated that, integration of 50 and 75% of the recommended dose of exogenous nitrogen fertiliser (i.e., 40 kg N ha-1 and 60 kg N ha-1) is the optimum level when the rhizobacteria, A. chroococcum results better in vivo nitrate reductase activity in the leaves, in vivo nitrogenase (N2-ase) activity on the root surface and viable counts, i.e., colony forming units (CFU) of microorganisms of rhizosphere soil of inoculated plants. Further increase of exogenous nitrogen inhibited both the nitrate reductase and nitrogenase activity and also decreased the population of microorganisms from rhizosphere soil.

Keywords: Azotobacter chroococcum,Colony forming units (CFU),Exogenous nitrogen,Nitrate reductase activity,Nitrogenase activity
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How to cite this article:

Roy, M., Saha, S., Das, J., 2017. Sensitivity of Azotobacter chroococcum to exogenous nitrogen in rice cultivation in terms of nitrate reductase activity in leaves and nitrogenase activity on the root surface of the inoculated rice cultivar and its effect on microorganism population in rhizosphere soil.Int.J.Curr.Res.Biosci.Plantbiol. 4(2): 54-59. doi: http://dx.doi.org/10.20546/ijcrbp.2017.402.007
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