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2 Dept. of Physiology, University of Kalyani, Environmental Physiology Division, Microbiology Unit, Kalyani, Nadia, West Bengal-741 235, India
The aim of this study was green synthesis of silver nanoparticles by using mint plant leaves extracts and to evaluate their antimicrobial activity. The fresh suspension of plant extracts was yellowish-green in colour. However, after addition of AgNO3 within 20mins, the suspension showed change in colour and turned dark brown after 5 hrs of incubation at room temperature. Formation of silver nanoparticles was confirmed using UV-Vis spectral analysis and showed silver surface plasmon resonance band in the range of 200-600 nm. It is a well-known fact, that silver ions and nanoparticles are highly toxic and hazardous to microorganisms. It is found out that the silver nanoparticles have many inhibitory and bactericidal effects and so its application is extended as an antibacterial agent. The antibacterial activity of silver nanoparticles is estimated by the zone of inhibition. The silver nanoparticles biosynthesized by mint plant leaves extracts showed antibacterial activity against microorganisms Escherichia coli, Pseudomonas aeruginosa, Baccillus subtilis (Gram Negative) and Staphylococcus aureus (Gram Positive). However, the antimicrobial effect varied with the variation in salt concentration. Additionally, the silver nanoparticles by mint plant leaves extracts showed good inhibition activity towards Escherichia coli and Pseudomonas aeruginosa. The use of silver nanoparticles in drug delivery systems might be the future thrust in the field of medicine.