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Abstract                 Volume:4  Issue-1  Year-2017          Original Research Articles

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Influence of Mercuric Chloride on Survival and Suitability for In Vitro Regeneration of Three Sweet Potato Landraces (Ipomoea batatas L.) Produced in Benin
Arsène M. Doussoh 1*, Justine Dangou-Sossou2, Serge S. Houédjissin1, Gilles H. T. Cacaï1,Armel K. Assogba1 and Corneille Ahanhanzo1
1Central Laboratory of Plant Biotechnology and Plant Breeding, Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP 526, Cotonou, Republic of Benin
2Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 BP 2009, Cotonou, Republic of Benin
*Corresponding author
Abstract:

Ipomoea batatas L. is tuberous root plant of great nutritional and economic importance in Benin. In vitro culture of this plant allows in a short period time, to produce a planting material through the establishment of efficient disinfection and micropropagation protocol. This work aims to study the survival and in vitro regeneration of three sweet potato landraces ('Dokoun', 'Vobodouaho' and 'Djlodou'). Four doses of mercuric chloride (C1: 0.05%, C2: 0.1%, C3: 0.5%, C4: 1%) were tested for disinfection and two Murashige and Skoog (MS) media, M0 (MS without growth regulator) and M1 (MS + 1mg/l of BAP + 0.01 mg/l of NAA) was used to evaluate the regeneration of the plantlets. The results showed that 0.5% mercuric chloride had the best survival rate (83.33% for 'Djlodou', 61.08% for 'Vobodouaho' and 52.75% for 'Dokoun'). Of the two media used, M0 has more adapted to landraces 'Vobodouaho' and 'Dokoun' while M1 contributed to a better development of the landraces 'Djlodou'.

Keywords: In vitro regeneration,Ipomoea batatas,Landraces,Mercuric chloride,
MS medium
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How to cite this article:

Doussoh, A. M., Dangou-Sossou, J., Houédjissin, S. S., Cacaï, G. H. T., Assogba, A. K., Ahanhanzo, C., 2017. Influence of mercuric chloride on survival and suitability for in vitro regeneration of three sweet potato landraces (Ipomoea batatas L.) produced in Benin.Int.J.Curr.Res.Biosci.Plantbiol. 4(1): 56-64. doi: http://dx.doi.org/10.20546/ijcrbp.2017.401.007
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