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Abstract                 Volume:3  Issue-12  Year-2016          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorinchiefijcrbp@gmail.com

Cloning and Sequence Analysis of a Squalene Synthase Gene from Ginkgo biloba
Liangqiong Ma, Li Zhu, Fang Xu, Weiwei Zhang* and Feng Xu
College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
*Corresponding author

To obtain the key genes for terpenoid saponins biosynthesis, a new squalene synthase gene was isolated by PCR from the leaves of Ginkgo biloba, named GbSS. And we also predicted the structure and physicochemical property of squalene synthase protein. The length of GbSS gene cDNA is 1998 bp, encoding 410 amino acids. The deduced protein sequence of GbSS has high homology with squalene synthase proteins from other plants, especially the gymnosperms. The putative GbSS protein is a hydrophilic protein and contains one putative transmembrane helices, it may be located in the plasma membrane and does not contain a signal peptide. Phylogenetic analysis revealed that the GbSS was closely related to TcSS, PmSS clustered into a single group, suggesting GbSS and squalene synthase from other Gymnosperms plants may be the same ancestor. These results provided a basis to further analyze the mechanism of terpenoid saponins biosynthesis of Ginkgo biloba.

Keywords: Ginkgo biloba,Squalene synthase,Terpenoid saponins,Sequence analysis
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How to cite this article:

Ma, L., Li, Z., Xu, F., Zhang, W., Xu, F., 2016. Cloning and sequence analysis of a squalene synthase gene from Ginkgo bilobaInt.J.Curr.Res.Biosci.Plantbiol. 3(12): 36-43. doi: http://dx.doi.org/10.20546/ijcrbp.2016.312.005
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