Abstract                 Volume:3  Issue-11  Year-2016          Original Research Articles

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Elicitation of Flavonoids in Blumea lacera (Burm.f.) DC. Cell Culture using Chemical Elicitor, Salicylic Acid and Biological Elicitor, Aspergillus niger
Vijay D. Mendhulkar*, Priyanka Patade and Moinuddin Vakil
Department of Botany, The Institute of Science, 15, Madam Cama Road, Mumbai- 400 032, Maharashtra, India
*Corresponding author
Abstract:

The present work was aimed to elicit flavonoid synthesis in cultured cells of Blumea lacera (Burm.f.) DC using Salicylic acid and Aspergillus niger as chemical and fungal elicitor respectively. Total flavonoid content in cultured cells was determined by Aluminum chloride colorimetric method. The 1.5 mM salicylic acid treatment for 24 hrs duration was highly responsive to elicit the flavonoid content (0.098 mg/g) as maximum as 2.8 fold over control (0.035 mg/g). In case of biological elicitor, Aspergillus niger treatment with 1.5 ml concentration for 4 days duration revealed 3.3 fold enhancement in flavonoid content (0.036 mg/g) as compared to control (0.011 mg/g). The results indicate that for flavonoid elicitation in Blumea lacera, Aspergillus niger is more responsive than Salicylic acid.

Keywords: Aspergillus niger,Blumea lacera,Cell culture,Flavonoids,Salicylic acid
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How to cite this article:

Mendhulkar, V. D., Patade, P., Vakil, M., 2016. Elicitation of flavonoids in Blumea lacera (Burm.f.) DC. cell culture using chemical elicitor, salicylic acid and biological elicitor, Aspergillus niger.Int.J.Curr.Res.Biosci.Plantbiol. 3(11): 85-91. doi: http://dx.doi.org/10.20546/ijcrbp.2016.311.013