|Online ISSN : 2349-8080
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2Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Chennai-600 024, Tamil Nadu, India
In the present study, the peroxidase enzyme was extracted from the tubers of Raphanus sativus L. and tested for their decolourization efficiency against reactive dyes. The extracted peroxidase was concentrated and purified by ion exchange chromatography. The optimum pH and temperature for the activity of the purified enzyme was found to be 6 and 37°C respectively. The molecular weight of the enzyme was determined as 44 kDa by SDS-PAGE analysis. The decolourization ability of peroxidase enzyme against dyes such as, Reactive blue 19 and Reactive yellow 12 were investigated. Maximum decolourization using enzyme was observed at pH 6 and temperature 37°C. The decolourization efficiency of the enzyme in the raw textile effluent was also determined. These present results suggest that the peroxidase extracted from R. sativus has great potential in the treatment of dyes present as pollutants in textile effluents.