Abstract                 Volume:3  Issue-11  Year-2016          Original Research Articles

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Analysis of Genetic Diversity of Dioscorea japonica Germplasm in China Using Inter-simple Sequence Repeat Markers
Jinping Wu, Zili Ding, Zhenbiao Jiao, Leifu Chen, Zhengming Qiu and Fengling Guo*
Hubei Academy of Agricultural Sciences, Wuhan 430064, China
*Corresponding author
Abstract:

Yams (Dioscorea spp.) are widely cultivated in China, with many landraces maintained by local farmers. However, little is known about their diversity or species identity. In this study, inter-simple sequence repeats (ISSRs) were used to determine genetic diversity within 64 yam landraces from 12 provinces of China. A total of 45 bands were amplified with five ISSR primers, of which 40 (88.89%) were polymorphic, suggesting a high level of polymorphism. Moreover, genetic diversity, estimated using Shannon’ index, was 0.3702, indicating relatively high genetic variation. A dendrogram of within-group linkage subsequently divided the 64 cultivars into three main clusters. Overall, the results suggest that these Chinese yam landraces are a valuable source of genes for future yam breeding programs

Keywords: Chinese yam,Dioscorea species,Genetic diversity,Inter-simple sequence repeats
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How to cite this article:

Wu, J., Ding, Z., Jiao, Z., Chen, L., Qiu, Z., Guo, F., 2016. Analysis of genetic diversity of Dioscorea japonica germplasm in China using inter-simple sequence repeat markers.Int.J.Curr.Res.Biosci.Plantbiol. 3(11): 36-42. doi: http://dx.doi.org/10.20546/ijcrbp.2016.311.006