Abstract                 Volume:3  Issue-11  Year-2016          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorinchiefijcrbp@gmail.com

Cloning and Sequence Analysis of Mevalonate Kinase Gene (CnMVK) from Chamaemelum nobile
Xiangxiang Meng, Weiwei Zhang, Feng Xu*, Jiaping Yan, Xiaomeng Liu, Yong-ling Liao and Jie Chang
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
*Corresponding author
Abstract:

Mevalonate kinase (MVK) is one of the key rate-limiting enzymes in mevalonic (MVA) pathway of terpenoid biosynthesis. To analyse the function of MVK gene in terpenoid biosynthesis in Chamaemelum nobile, the primers were designed according to the transcript unique sequence of CnMVK from the C. nobile transcriptome dataset. A MVK gene (designated as CnMVK, GenBank accession number KX894317) was cloned from C. nobile using RT-PCR method. The full-length cDNA of CnMVK gene is 1561bp and contains an open reading frame (ORF) of 1206bp, which encoding a 402 amino-acid protein. The theoretical molecular weight and pI of the CnMVK are 42.8kDa and 5.79, respectively. Multi-alignment comparison analysis showed the protein sequence of CnMVK had high similarity with MVK proteins from other plants. Furthermore, CnMVK has similar conserved domains, suggesting CnMVK is one of MVK family mumbers in C. nobile. The present study cloned, analyzed the sequence of CnMVK in C. nobile will provide a foundation for exploring the molecular mechanism of terpenoid biosynthesis in C. nobile.

Keywords: Chamaemelum nobile,CnMVK gene,Gene cloning,Sequence analysis
Download this article as Download

How to cite this article:

Meng, X., Zhang, W., Xu, F., Yan, J., Liu, X., Liao, Y., Chang, J., 2016. Cloning and sequence analysis of mevalonate kinase gene (CnMVK) from Chamaemelum nobile.Int.J.Curr.Res.Biosci.Plantbiol. 3(11): 23-28. doi: http://dx.doi.org/10.20546/ijcrbp.2016.311.004