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Abstract                 Volume:3  Issue-10  Year-2016          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
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Determination of Acquaintance between Biofilm and Extended Spectrum of β-Lactamases Producers from Diarrheal Stool Isolates of Escherichia coli
D. Jegadeeshkumar1, K. S. Rajen2, P. Nirmala3, L. R. Gopinath3 and B. Prakash3*
1Reseach Scholar, Manonmaniam Sundaranar University, Tirunelveli-627 012, Tamil Nadu, India
2Department of Microbiology, Mannai Rajagopalasamy Government Arts College, Mannargudi- 614 001, Tamil Nadu, India
3Department of Biotechnology, Vivekanandha College of Arts & Science for Women, Tiruchengode- 637 205, Tamil Nadu, India
*Corresponding author
Abstract:

The study was to assess the prevalence of extended spectrum beta-lactamases (ESBL) positive isolates from diarrheal stool samples in Namakkal District, Tamil Nadu (India). Totally 68 isolates were recovered from 125 samples and the isolates were identified as Escherichia coli by biochemical test and selective media. All isolates were subjected to determination of biofilm formation with tube method, among them 36 (53%) isolates were biofilm producers. The ESBL production was detected using a double disc synergy test and the presence of ESBL genes was evaluated by the Multiplex PCR method. In this study, 32.3% of isolates has been at least one type of ESBL genes, among them CTXm was most predominate gene. This result was acquainted with biofilm producers, and it was found that biofilm producing isolates mostly harbored the ESBL genes in comparison with non-biofilm producers.

Keywords: Biofilm, CTXm, ESBL genes, Escherichia coli
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How to cite this article:

Jegadeeshkumar, D., Rajen, K. S., Nirmala, P., Gopinath, L. R., Prakash, B., 2016. Determination of acquaintance between biofilm and extended spectrum β-lactamases producers from diarrheal stool isolates of Escherichia coli.Int.J.Curr.Res.Biosci.Plantbiol. 3(10): 174-178. doi: http://dx.doi.org/10.20546/ijcrbp.2016.310.022
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