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Abstract                 Volume:3  Issue-10  Year-2016          Original Research Articles

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Molecular Cloning and Sequence Analysis of a Phosphomevalonate Kinase Gene (CnPMK) from Chamaemelum nobile
Jiaping Yan, Xiangxiang Meng, Feng Xu* and Jie Chang*
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
*Corresponding author

Roman chamomile (Chamaemelum nobile L.) is renowned for its production of sesquiterpenoids, and specifically sesquiterpene lactones. Phosphomevalonate Kinase gene (PMK) is one of the core enzymes in the biosynthesis pathway of sesquiterpenoids, which catalyzes the formation ofmevalonate-5-diphosphate. To isolate and identify the key genes involved in the sesquiterpenoids biosynthesis of the C. nobile, a PMK gene designated as CnPMK (GenBank Accession No. KX894316) was cloned from C. nobile. The full-length cDNA of CnPMK was 1942bp and contained a 1482bp open reading frame (ORF), which encoding a 493 amino-acid protein. The molecular weight and pI of the CnPMK protein are 53.8KDa and 5.55, respectively. The encoding amino-acid sequence of the CnGAS showed high similarity to other plant PMK proteins. Phylogenetic tree analysis revealed that CnPMK clustered with the PMK of Asteraceae in the dicotyledons clade. The CnPMK gene was isolated from C. nobile, which will lay a foundation for further study of sesquiterpenoid biosynthesis pathway in C. nobile.

Keywords: Chamaemelum nobile,Molecular cloning,Phosphomevalonate kinase,Sesquiterpene biosynthesis
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How to cite this article:

Yan, J., Meng, X., Xu, F., Chang, J., 2016. Molecular cloning and sequence analysis of a phosphomevalonate kinase gene (CnPMK) from Chamaemelum nobile.Int.J.Curr.Res.Biosci.Plantbiol. 3(10): 157-162. doi: http://dx.doi.org/10.20546/ijcrbp.2016.310.019
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