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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
Roman chamomile (Chamaemelum nobile L.) is renowned for its production of sesquiterpenoids, and specifically sesquiterpene lactones. Phosphomevalonate Kinase gene (PMK) is one of the core enzymes in the biosynthesis pathway of sesquiterpenoids, which catalyzes the formation ofmevalonate-5-diphosphate. To isolate and identify the key genes involved in the sesquiterpenoids biosynthesis of the C. nobile, a PMK gene designated as CnPMK (GenBank Accession No. KX894316) was cloned from C. nobile. The full-length cDNA of CnPMK was 1942bp and contained a 1482bp open reading frame (ORF), which encoding a 493 amino-acid protein. The molecular weight and pI of the CnPMK protein are 53.8KDa and 5.55, respectively. The encoding amino-acid sequence of the CnGAS showed high similarity to other plant PMK proteins. Phylogenetic tree analysis revealed that CnPMK clustered with the PMK of Asteraceae in the dicotyledons clade. The CnPMK gene was isolated from C. nobile, which will lay a foundation for further study of sesquiterpenoid biosynthesis pathway in C. nobile.
