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a,bThe authors contributed equally to this study
Anthocyanins are biosynthesized through a series of enzymes with the transcript levels of their encoding genes commonly regulated by the MBW transcription complex containing MYB, bHLH and WD40 transcription factors. As one of the largest plant transcription factor families, MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factors play an important role in plant anthocyanin biosynthetic pathway. In order to investigate the relationship MYB transcription factors of peach peel, a transcription factor gene MYB44 were isolated from Prunus persica by using RT-PCR technology. Sequence analysis indicated MYB44 that were 669bp, encoding 223 amino acids, with a calculated molecular mass of 25.68kDa and an isoelectric point of 9.06, which was designated as PpMYB44 (GenBank accession No. KX852400). Bioinformatics analysis showed that the deduced PpMYB44, which shared more than 60% identities with the MYB protein from other plants. The protein predication of PpMYB44 showed that there were a function domains MYB_DNA-binding and typical structure of SANT superfamily. Predicted protein secondary structure showed that PpMYB44 protein contents of the structure with alpha helix (Hh) 35.59%, extended strand (Ee) 19.82%, random coil (Cc) 35.58%, and beta turn (Tt) 9.01%, it had high similarities with Malus domestica, Pyrus×bretschneideri, and Medicago truncatula MYB proteins. Phylogenetic analysis results revealed that PpMYB44 were closely related to anthocyanin biosynthesis MYB transcription factors in Prunus mume, Malus domestica, and Pyrus×bretschneideri. These results indicate that PpMYB44 belongs to MYB families and responsible for anthocyanin biosynthetic in peach peels.