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Department of Microbiology, Kanchi Shri Krishna College of Arts and Science, Kilambi, Kancheepuram-631 551, Tamil Nadu, India
Acetone, ethanol, methanol and petroleum ether leaf extracts of the plants, Euphorbia heterophylla and Tamilnadia uliginosa were tested for their antibacterial activity against Xanthomonas campestris pv. citri using disc diffusion technique. The leaf extract concentrations tested were 0 (control), 125, 250, 500 and 1000 ppm for each plant. The control (0 ppm) received no extracts and chloramphenicol (30 µg) was used as standard antibiotic. The antibacterial activity of the extracts used in the present study was found to be concentration dependent. The highest zone of inhibition, 2.8 cm was recorded in 1000ppm ethanolic leaf extract of Tamilnadia uliginosa with an activity index of 0.90 against Xanthomonas campestris pv. citri. At 125 ppm concentration, ethanolic leaf extracts of Euphorbia heterophylla showed zone of inhibition (0.8 cm) and the other solvent extracts showed no activity. However, at 125 ppm concentration, acetone, ethanol and methanol leaf extracts of Tamilnadia uliginosa showed least inhibition zones, 1.2 and 0.8 cm respectively.