Abstract                 Volume:3  Issue-8  Year-2016          Original Research Articles

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Antioxidant Activity of Phycobiliproteins from Dermonema virens (J.Agardh) Pedroche & Ávila Ortiz (Rhodophyta: Liagoraceae) on Lactuca sativa L. Seedlings
Saraí Eunice Ortiz García, Roxana Olvera Ramírez and Nora Beatriz Medina Jaritz*
Laboratorio de Fisiología Vegetal, Departamento de Botánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City 11340, México
*Corresponding author
Abstract:

Live beings have shields against oxidative damage from reactive oxygen (ROS). These shields are the antioxidant systems and can be enzymatic or non-enzymatic. The phycobiliproteins are part of the non-enzymatic systems and they are water soluble photosynthetic pigments found in cyanobacteria and red algae. They can scavenge free radicals. Phycobiliprotein extract from seaweed Dermonema virens was performed macerating it in liquid N2, then the biomass was suspended in water and centrifuged, and finally the supernatant spectrophotometrically characterized. Lactuca sativa seeds were exposed to three different media to evaluate germination and elongation of the radicle and hypocotyl. A high concentration of phycoerythrin was found, with an absorption peak at 550 nm. In trials with water, 4-6 cm growth in radicle and hypocotyl was obtained, while the ROS caused necrosis in the radicle and reached just 10% of germination. Testing the extract in the presence of ROS, seed germination was at 90% and radicle from 1 to 3 cm. It is concluded that the extract has antioxidant activity that protects lettuce seeds from oxidative damage from ROS.

Keywords: Antioxidants,Dermonema virens,Lactuca sativa,Phycobiliproteins
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How to cite this article:

Ortiz García, S. E., Olvera Ramírez, S., Medina Jaritz, N. B., 2016. Antioxidant activity of phycobiliproteins from Dermonema virens (J. Agardh) Pedroche & Ávila Ortiz (Rhodophyta: Liagoraceae) on Lactuca sativa L. seedlings.Int.J.Curr.Res.Biosci.Plantbiol. 3(8): 19-24. doi: http://dx.doi.org/10.20546/ijcrbp.2016.308.003